JFLEX / Janus Investment Fund - Janus Henderson Flexible Bond Fund Class I - Pengajuan SECLaporan Tahunan, Pernyataan Proksi

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CORRESP [ Janus Henderson Investors US LLC Letterhead ] June 17, 2025 VIA EDGAR Samantha Brutlag Division of Investment Management U.

N-MFP3

N-MFP3 0000277751 XXXXXXXX 2025-02-28 JANUS INVESTMENT FUND 0000277751 W6UYDZFUOFJ4HQQ32382 Janus Henderson Government Money Market 213800N2U69WG3AIGH81 S000010466 4 N N 002-34393 JANUS HENDERSON INVESTORS US LLC 801-13991 PRICEWATERHOUSECOOPERS LLP DENVER CO JANUS HENDERSON INVESTORS US LLC JANUS HENDERSON SERVICES US LLC 0001170078 084-06024 N N N Government N N 29 29 209491915.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒       Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒         Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2))

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

DEFA14A 1 d836570ddefa14a.htm DEFA14A UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Defin

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Defin

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Defin

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Schedule 14(a) of the Securities and Exchange Act of 1934 (Amendment No. )

Table of Contents UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Schedule 14(a) of the Securities and Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Ru

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[ Janus Henderson Investors US LLC Letterhead ] November 4, 2024 VIA EDGAR Jennifer McHugh Division of Investment Management U.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Schedule 14(a) of the Securities and Exchange Act of 1934 (Amendment No. )

Table of Contents UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Schedule 14(a) of the Securities and Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☒ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Ru

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[ Janus Henderson Investors US LLC Letterhead ] July 2, 2024 VIA EDGAR Jeff Foor Division of Investment Management U.

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NPORT-EX 2 nportex-202.htm Janus Henderson Global Allocation Fund - Growth Schedule of Investments (unaudited) March 31, 2023 Shares Value Investment Companies£– 100.0% Equity Funds – 87.4% Janus Henderson Adaptive Global Allocation Fund - Class N Shares 1,265,535 $12,098,263 Janus Henderson Adaptive Risk Managed U.S. Equity Fund - Class N Shares 1,821,572 16,885,459 Janus Henderson Asia Equity Fu

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NPORT-EX 2 nportex-04.htm Janus Henderson Flexible Bond Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 21.8% 208 Park Avenue Mortgage Trust 2017-280P, ICE LIBOR USD 1 Month + 0.8800%, 5.5990%, 9/15/34 (144A)‡ $4,050,930 $3,932,313 ACC Auto Trust 2021-A A, 1.0800%, 4/15/27 (144A) 497,058 493,158 ACC Auto

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NPORT-EX 2 nportex-142.htm Janus Henderson Small-Mid Cap Value Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Common Stocks– 97.3% Aerospace & Defense – 1.9% BWX Technologies Inc 30,966 $1,952,097 Auto Components – 1.8% Aptiv PLC* 16,615 1,864,037 Banks – 7.4% Ameris Bancorp 47,955 1,754,194 First Interstate BancSystem Inc - Class A 66,505 1,985,839 Synov

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Janus Henderson Global Allocation Fund - Moderate Schedule of Investments (unaudited) March 31, 2023 Shares Value Investment Companies£– 100.

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NPORT-EX 2 nportex-100.htm Janus Henderson Small Cap Value Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Common Stocks– 96.6% Aerospace & Defense – 2.6% AAR Corp* 347,402 $18,950,779 AerSale Corp* 1,348,845 23,227,111 Spirit AeroSystems Holdings Inc 671,863 23,199,429 65,377,319 Auto Components – 1.8% Adient PLC* 578,691 23,703,183 Dorman Products Inc* 2

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NPORT-EX 2 nportex-102.htm Janus Henderson Mid Cap Value Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Common Stocks– 98.4% Aerospace & Defense – 3.3% BWX Technologies Inc 417,672 $26,330,043 L3Harris Technologies Inc 203,231 39,882,051 66,212,094 Auto Components – 2.0% Aptiv PLC* 185,686 20,832,112 Autoliv Inc 215,445 20,113,945 40,946,057 Banks – 3.9%

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NPORT-EX 2 nportex-57.htm Janus Henderson High-Yield Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 2.6% Amercian Airlines Inc / AAdvantage Loyalty IP Ltd, 5.5000%, 4/20/26 (144A) $2,969,734 $2,922,550 BX Commercial Mortgage Trust 2021-ARIA F, ICE LIBOR USD 1 Month + 2.5935%, 7.2775%, 10/15/36 (144A)‡ 6,

GBP

NPORT-EX 2 nportex-164.htm Janus Henderson Developed World Bond Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.3% Tesco Property Finance 3 PLC, 5.7440%, 4/13/40((cost $6,729,817) 3,690,752 GBP $4,487,622 Corporate Bonds– 48.6% Banking – 7.8% Bank of America Corp, 4.2000%, 8/26/24 $1,860,000 1,827,410 B

GBP

NPORT-EX 2 nportex-135.htm Janus Henderson Global Bond Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 1.0% JP Morgan Mortgage Trust 2019-1 A15, 4.0000%, 5/25/49 (144A)‡ $110,578 $100,102 JP Morgan Mortgage Trust 2019-LTV2, 3.5000%, 12/25/49 (144A)‡ 8,848 8,754 RESIMAC Bastille Trust Series 2018-1NC, ICE

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NPORT-EX 2 nportex-157.htm Janus Henderson Responsible International Dividend Fund Schedule of Investments (unaudited) March 31, 2023 Shares Value Common Stocks– 94.7% Automobiles – 2.3% Stellantis NV 142,984 $2,601,487 Banks – 4.0% BAWAG Group AG (144A)* 45,893 2,231,340 ING Groep NV 195,814 2,329,397 4,560,737 Beverages – 1.6% Coca-Cola Co 29,548 1,832,862 Capital Markets – 1.0% CME Group Inc 6,

CAD

Janus Henderson Multi-Sector Income Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 59.

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NPORT-EX 2 nportex-201.htm Janus Henderson Global Allocation Fund - Conservative Schedule of Investments (unaudited) March 31, 2023 Shares Value Investment Companies£– 100.0% Equity Funds – 46.2% Janus Henderson Adaptive Global Allocation Fund - Class N Shares 859,120 $8,212,845 Janus Henderson Adaptive Risk Managed U.S. Equity Fund - Class N Shares 682,043 6,321,685 Janus Henderson Asia Equity Fu

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NPORT-EX 2 nportex-119.htm Janus Henderson Adaptive Risk Managed U.S. Equity Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Common Stocks– 98.6% Aerospace & Defense – 2.0% General Dynamics Corp 28,972 $6,611,700 L3Harris Technologies Inc 28,618 5,615,996 12,227,696 Auto Components – 0.4% BorgWarner Inc 56,215 2,760,719 Automobiles – 0.7% Tesla Inc* 21,940

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NPORT-EX 2 nportex-11.htm Janus Henderson Short Duration Flexible Bond Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 39.6% 208 Park Avenue Mortgage Trust 2017-280P, ICE LIBOR USD 1 Month + 0.8800%, 5.5990%, 9/15/34 (144A)‡ $2,890,000 $2,805,377 ACC Auto Trust 2021-A A, 1.0800%, 4/15/27 (144A) 145,978 14

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NPORT-EX 2 nportex-170.htm Janus Henderson Sustainable Multi-Asset Allocation Fund Schedule of Investments (unaudited) March 31, 2023 Shares Value Investment Companies£– 104.8% Exchange-Traded Funds (ETFs) – 96.9% Janus Henderson International Sustainable Equity 33,107 $599,237 Janus Henderson Net Zero Transition Resources 12,669 295,425 Janus Henderson Sustainable & Impact Core Bond 28,858 1,236,

AUD

NPORT-EX 2 nportex-148.htm Janus Henderson Absolute Return Income Opportunities Fund Schedule of Investments (unaudited) March 31, 2023 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 2.9% Connecticut Avenue Securities Trust 2022-R01 1M1, US 30 Day Average SOFR + 1.0000%, 5.5598%, 12/25/41 (144A)‡ $182,387 $180,163 Connecticut Avenue Securities Trust 2022-R08

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NPORT-EX 2 nportex-152.htm Janus Henderson Adaptive Global Allocation Fund Schedule of Investments (unaudited) March 31, 2023 Shares/Principal/ Contract Amounts Value Investment Companies– 6.5% Exchange-Traded Funds (ETFs) – 6.5% Vanguard Intermediate-Term Corporate Bond 26,206 $2,102,245 Vanguard Long-Term Corporate Bond 5,824 464,289 Total Investment Companies (cost $2,478,110) 2,566,534 Commerc

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

Form DEFA14A UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

Form DEFA14A UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

Form DEFA14A UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

Form DEFA14A UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. )

Form DEFA14A UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e

[ Janus Henderson Letterhead ]

[ Janus Henderson Letterhead ] March 8, 2023 EDGAR Operations Branch Securities and Exchange Commission Division of Investment Management 100 F Street, N.

Expense Limitation Agreement between Janus Henderson Investors US LLC and Janus Investment Fund, regarding Janus Henderson Overseas Fund, is filed herein as Exhibit 13(m).

Expense Limitation Agreement for Janus Henderson Overseas Fund Exhibit 13(m) EXPENSE LIMITATION AGREEMENT JANUS INVESTMENT FUND JANUS HENDERSON OVERSEAS FUND March 6, 2023 Janus Investment Fund 151 Detroit Street Denver, Colorado 80206 Ladies and Gentlemen: As you know, Section 5 of our Investment Advisory Agreement provides for compensation payable to Janus Henderson Investors US LLC (the “Adviser”) with respect to Janus Henderson Overseas Fund (the “Fund”).

Consent of PricewaterhouseCoopers LLP is filed herein as Exhibit 14(a).

Consent of PricewaterhouseCoopers LLP Exhibit 14(a) CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM We hereby consent to the incorporation by reference in this Registration Statement on Form N-14 of Janus Investment Fund of our reports dated November 28, 2022, relating to the financial statements and financial highlights, which appear in Janus Henderson International Opportunities Fund and Janus Henderson Overseas Fund’s Annual Report on Form N-CSR for the year ended September 30, 2022.

Opinion and Consent of Counsel is filed herein as Exhibit 11(a).

Opinion and Consent of Counsel Exhibit 11(a) March 7, 2023 Janus Investment Fund 151 Detroit Street Denver, CO 80206-4805 Re: Janus Investment Fund Registration Statement on Form N-14 Ladies and Gentlemen: I have acted as Vice President, Secretary, and Chief Legal Counsel for Janus Investment Fund, a Massachusetts business trust (the “Trust”), in connection with the registration statement on Form N-14 (File No.

As filed with the Securities and Exchange Commission on March 8, 2023

N-14/A Table of Contents As filed with the Securities and Exchange Commission on March 8, 2023 Securities Act File No.

Expense Limitation Agreement between Janus Henderson Investors US LLC and Janus Investment Fund, regarding certain series with a September 30 fiscal year end, is filed herein as Exhibit 13(k).

Expense Limitation Agreement for certain series with a Sept 30 Fiscal Year End Exhibit 13(k) EXPENSE LIMITATION AGREEMENT JANUS INVESTMENT FUND September 30 Funds December 14, 2022 Janus Investment Fund 151 Detroit Street Denver, Colorado 80206 Ladies and Gentlemen: As you know, Section 5 of our Investment Advisory Agreement provides for compensation payable to Janus Henderson Investors US LLC (the “Adviser”) with respect to the funds listed in Schedule A (collectively, the “Funds” and each, a “Fund”).

Non-Standard Waiver Agreement between Janus Henderson Investors US LLC and Janus Investment Fund, regarding Janus Henderson Overseas Fund, is filed herein as Exhibit 13(l).

Non-Standard Waiver Agreement Exhibit 13(l) NON-STANDARD WAIVER AGREEMENT JANUS HENDERSON OVERSEAS FUND March 7, 2023 Janus Investment Fund 151 Detroit Street Denver, Colorado 80206 Ladies and Gentlemen: As you know, Section 5 of our Investment Advisory Agreement provides for compensation payable to Janus Henderson Investors US LLC (the “Adviser”) with respect to Janus Henderson Overseas Fund (the “Fund”).

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CORRESP [ Janus Henderson Investors US LLC Letterhead ] March 6, 2023 VIA EDGAR Samantha Brutlag Christina Fettig Division of Investment Management U.

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NPORT-EX 2 nportex-139.htm Janus Henderson Asia Equity Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 99.0% Automobiles – 2.6% BYD Co Ltd 19,000 $467,262 Eicher Motors Ltd 5,713 222,074 689,336 Banks – 11.8% Bank Negara Indonesia Persero Tbk PT 1,306,400 771,347 HDFC Bank Ltd 40,433 795,985 ICICI Bank Ltd 103,911 1,113,837 Vietnam Technological & Commercial

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NPORT-EX 2 nportex-08.htm Janus Henderson Balanced Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 8.7% 208 Park Avenue Mortgage Trust 2017-280P, ICE LIBOR USD 1 Month + 0.8800%, 5.1170%, 9/15/34 (144A)‡ $11,417,538 $11,138,387 ACC Auto Trust 2022-A A, 4.5800%, 7/15/26 (144A) 7,294,096 7,158,261 ACM Au

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Janus Henderson U.S. Dividend Income Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 99.8% Aerospace & Defense – 2.1% General Dynamics Corp 71 $17,616 Air Freight & Logistics – 3.2% United Parcel Service Inc 159 27,641 Banks – 8.3% Bank of America Corp 386 12,784 JPMorgan Chase & Co 248 33,257 US Bancorp 580 25,294 71,335 Beverages – 4.0% Coca-Cola Co 227 14,

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NPORT-EX 2 nportex-03.htm Janus Henderson Venture Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 97.9% Auto Components – 0.8% Quantumscape Corp*,# 486,572 $2,758,863 Visteon Corp* 158,240 20,702,539 23,461,402 Automobiles – 0.5% Thor Industries Inc 202,891 15,316,242 Banks – 0.9% Bancorp Inc/The* 927,645 26,326,565 Biotechnology – 9.3% A

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NPORT-EX 2 nportex-113.htm Janus Henderson Triton Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 97.5% Aerospace & Defense – 1.9% Aerojet Rocketdyne Holdings Inc* 669,428 $37,441,108 Axon Enterprise Inc* 570,924 94,733,419 132,174,527 Auto Components – 1.2% Fox Factory Holding Corp* 234,200 21,366,066 Quantumscape Corp*,# 1,293,635 7,334

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NPORT-EX 2 nportex-159.htm Janus Henderson European Focus Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 96.0% Aerospace & Defense – 2.7% Rheinmetall AG 15,044 $2,996,110 Safran SA 64,138 8,013,750 11,009,860 Automobiles – 0.8% Daimler AG 46,533 3,053,225 Banks – 8.2% BNP Paribas SA 110,746 6,301,362 Commerzbank AG* 539,146 5,097,405 FinecoBank Banca Fineco

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Janus Henderson Growth and Income Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 99.

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NPORT-EX 2 nportex-12.htm Janus Henderson Research Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 99.9% Aerospace & Defense – 2.1% General Dynamics Corp 622,170 $154,366,599 Howmet Aerospace Inc 3,755,644 148,009,930 302,376,529 Air Freight & Logistics – 2.5% United Parcel Service Inc 2,061,352 358,345,432 Automobiles – 0.9% Rivian Autom

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Janus Henderson Global Select Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 96.

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Janus Henderson Enterprise Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 98.

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Janus Henderson Emerging Markets Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 96.

[ Janus Henderson Investors US LLC Letterhead ]

DEL AM [ Janus Henderson Investors US LLC Letterhead ] March 1, 2023 VIA EDGAR Division of Investment Management U.

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Janus Henderson Global Research Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 98.

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NPORT-EX 2 nportex-127.htm Janus Henderson Global Real Estate Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 99.6% Diversified Telecommunication Services – 0.9% Cellnex Telecom SA (144A)* 163,791 $5,446,195 Equity Real Estate Investment Trusts (REITs) – 79.2% Activia Properties Inc 1,654 5,188,946 Advance Residence Investment Corp 1,697

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NPORT-EX 2 nportex-14.htm Janus Henderson Overseas Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 94.6% Aerospace & Defense – 2.3% Airbus SE 399,729 $47,521,919 Banks – 10.4% BNP Paribas SA 1,475,462 83,952,645 Erste Group Bank AG 1,859,363 59,282,183 Natwest Group PLC 18,738,763 59,803,987 Permanent TSB Group Holdings PLC* 7,893,218 15,

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NPORT-EX 2 nportex-72.htm Janus Henderson Global Technology and Innovation Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 97.9% Aerospace & Defense – 1.2% Axon Enterprise Inc* 278,358 $46,187,943 Electronic Equipment, Instruments & Components – 1.2% Amphenol Corp 458,312 34,895,876 E Ink Holdings Inc 1,912,000 10,006,744 44,902,620 Equity Real Estate Investm

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NPORT-EX 2 nportex-75.htm Janus Henderson Contrarian Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Corporate Bonds– 1.3% Consumer Cyclical – 1.3% Colt Merger Sub Inc, 5.7500%, 7/1/25 (144A)((cost $52,337,769) $53,270,000 $52,146,326 Common Stocks– 98.1% Aerospace & Defense – 5.5% Howmet Aerospace Inc 5,723,717 225,571,687 Banks – 2.8% Cullen/Frost Ban

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NPORT-EX 2 nportex-160.htm Janus Henderson Global Equity Income Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 93.9% Aerospace & Defense – 0.5% BAE Systems PLC 2,635,838 $27,236,093 Automobiles – 0.7% Stellantis NV* 3,056,347 43,286,790 Banks – 6.1% Hana Financial Group Inc 1,715,988 57,422,860 Intesa Sanpaolo SpA 25,378,171 56,610,374 KB Financial Group Inc

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Janus Henderson International Opportunities Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 99.

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NPORT-EX 2 nportex-167.htm Janus Henderson Global Sustainable Equity Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 97.9% Auto Components – 1.7% Aptiv PLC* 4,944 $460,435 Building Products – 1.0% Advanced Drainage Systems Inc 3,205 262,714 Containers & Packaging – 2.1% DS Smith PLC 139,834 543,386 Electric Utilities – 2.2% SSE PLC 28,104 579,547 Electrical E

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NPORT-EX 2 nportex-42.htm Janus Henderson Forty Fund Schedule of Investments (unaudited) December 31, 2022 Shares Value Common Stocks– 98.0% Aerospace & Defense – 1.7% Howmet Aerospace Inc 6,221,261 $245,179,896 Automobiles – 0.4% Rivian Automotive Inc - Class A* 2,941,451 54,210,942 Banks – 1.8% JPMorgan Chase & Co 1,870,525 250,837,403 Biotechnology – 3.6% AbbVie Inc 3,131,827 506,134,561 Capita

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NPORT-EX 2 nportex-71.htm Janus Henderson Global Life Sciences Fund Schedule of Investments (unaudited) December 31, 2022 Shares or Principal Amounts Value Common Stocks– 97.5% Biotechnology – 30.9% 4D Molecular Therapeutics Inc* 444,198 $9,865,638 AbbVie Inc 1,141,497 184,477,330 Akero Therapeutics Inc* 1,001,770 54,896,996 Alnylam Pharmaceuticals Inc* 172,691 41,040,016 Altimmune Inc*,# 1,030,77

Form of Agreement and Plan of Reorganization by Janus Investment Fund, on behalf of Janus Henderson International Opportunities Fund and Janus Henderson Overseas Fund, is filed herein as Exhibit 4(a).

Exhibit 4(a) FORM OF AGREEMENT AND PLAN OF REORGANIZATION THIS AGREEMENT AND PLAN OF REORGANIZATION (the “Agreement”) is made as of this [ ]th day of [ ], 2023, by and between Janus Investment Fund, a Massachusetts business trust (the “Trust”), on behalf of Janus Henderson International Opportunities Fund, a series of the Trust (the “Target Fund”), and Janus Henderson Overseas Fund, a series of the Trust (the “Acquiring Fund”).

Powers of Attorney, dated January 27, 2023, is filed herein as Exhibit 16(a).

Exhibit 16(a) JANUS INVESTMENT FUND (THE “TRUST”) POWER OF ATTORNEY KNOW ALL PERSONS BY THESE PRESENTS, the undersigned hereby makes, constitutes and appoints the President and Chief Executive Officer of the Trust; Vice President, Chief Legal Counsel, and Secretary of the Trust; and Chief Financial Officer, Vice President, Treasurer, and Principal Accounting Officer of the Trust, and each of them,

As filed with the Securities and Exchange Commission on January 31, 2023

N-14 Table of Contents As filed with the Securities and Exchange Commission on January 31, 2023 Securities Act File No.

Form of Non-Standard Waiver Agreement between Janus Henderson Investors US LLC and Janus Investment Fund, regarding Janus Henderson Overseas Fund, is filed herein as Exhibit 13(l).

Exhibit 13(l) FORM OF NON-STANDARD WAIVER AGREEMENT JANUS HENDERSON OVERSEAS FUND [ ], 2023 Janus Investment Fund 151 Detroit Street Denver, Colorado 80206 Ladies and Gentlemen: As you know, Section 5 of our Investment Advisory Agreement provides for compensation payable to Janus Henderson Investors US LLC (the “Adviser”) with respect to Janus Henderson Overseas Fund (the “Fund”).

2 Appendix A

[ Janus Henderson Investors US LLC Letterhead ] December 13, 2022 VIA EDGAR Deborah O’Neal Division of Investment Management U.

2

Janus Henderson Sustainable Multi-Asset Allocation Fund Schedule of Investments (unaudited) September 30, 2022 Shares Value Investment Companies£– 101.

2

Janus Henderson Short Duration Flexible Bond Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 35.

2

Janus Henderson Adaptive Global Allocation Fund Schedule of Investments (unaudited) September 30, 2022 Shares/Principal/ Contract Amounts Value Common Stocks– 19.

CAD

Janus Henderson Multi-Sector Income Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 61.

2

Janus Henderson Small Cap Value Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson Global Allocation Fund - Growth Schedule of Investments (unaudited) September 30, 2022 Shares Value Investment Companies£– 100.

GBP

Janus Henderson Global Bond Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 1.

GBP

Janus Henderson Developed World Bond Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

2

Janus Henderson High-Yield Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

2

Janus Henderson Global Allocation Fund - Conservative Schedule of Investments (unaudited) September 30, 2022 Shares Value Investment Companies£– 100.

2

Janus Henderson Dividend & Income Builder Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Corporate Bonds– 11.

2

Janus Henderson Mid Cap Value Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Flexible Bond Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 23.

2

Janus Henderson Global Allocation Fund - Moderate Schedule of Investments (unaudited) September 30, 2022 Shares Value Investment Companies£– 100.

AUD

Janus Henderson Absolute Return Income Opportunities Fund Schedule of Investments (unaudited) September 30, 2022 Shares/Principal/ Contract Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 3.

2

Janus Henderson Adaptive Risk Managed U.S. Equity Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Common Stocks– 97.8% Aerospace & Defense – 0.5% Howmet Aerospace Inc 103,077 $3,188,172 Auto Components – 0.4% BorgWarner Inc 78,376 2,461,006 Banks – 10.5% Bank of Nova Scotia# 103,168 4,907,702 Bank OZK 95,494 3,777,743 Canadian Imperial Bank of Commerce

2

Janus Henderson Small-Mid Cap Value Fund Schedule of Investments (unaudited) September 30, 2022 Shares or Principal Amounts Value Common Stocks– 98.

2

CORRESP 1 filename1.htm [ Janus Henderson Investors US LLC Letterhead ] October 20, 2022 VIA EDGAR Deborah O’Neal Division of Investment Management U.S. Securities and Exchange Commission 100 F Street, N.E. Washington, DC 20549-0505 Re: JANUS INVESTMENT FUND (the “Registrant”) 1933 Act File No. 002-34393 1940 Act File No. 811-01879 Post-Effective Amendment No. 311 Dear Ms. O’Neal: This letter resp

2

Janus Henderson Overseas Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 95.

2

Janus Henderson Global Life Sciences Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Forty Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Triton Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Global Technology and Innovation Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Research Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson International Opportunities Fund Schedule of Investments (unaudited) June 30, 2022 Shares Value Common Stocks– 99.

2

Janus Henderson Asia Equity Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson Global Research Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Global Sustainable Equity Fund Schedule of Investments (unaudited) June 30, 2022 Shares Value Common Stocks– 95.

2

NPORT-EX 2 nportex-3.htm Janus Henderson Venture Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 98.0% Auto Components – 0.9% Quantumscape Corp*,# 486,572 $4,179,653 Visteon Corp* 223,534 23,153,652 27,333,305 Automobiles – 0.6% Thor Industries Inc 212,739 15,897,985 Banks – 0.6% Bancorp Inc/The* 927,645 18,107,630 Biotechnology – 6.5% Ascend

2

Janus Henderson Contrarian Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson European Focus Fund Schedule of Investments (unaudited) June 30, 2022 Shares/Principal/ Contract Amounts Value Common Stocks– 94.

[Janus Henderson Letterhead]

[Janus Henderson Letterhead] August 29, 2022 EDGAR Operations Branch Securities and Exchange Commission Division of Investment Management 100 F Street, N.

2

Janus Henderson Global Real Estate Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Emerging Markets Fund Schedule of Investments (unaudited) June 30, 2022 Shares Value Common Stocks– 97.

2

Janus Henderson Global Equity Income Fund Schedule of Investments (unaudited) June 30, 2022 Shares Value Common Stocks– 95.

2

Janus Henderson Enterprise Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson Global Select Fund Schedule of Investments (unaudited) June 30, 2022 Shares Value Common Stocks– 98.

2

NPORT-EX 2 nportex-5.htm Janus Henderson Growth and Income Fund Schedule of Investments (unaudited) June 30, 2022 Shares Value Common Stocks– 100.1% Aerospace & Defense – 2.6% General Dynamics Corp 525,901 $116,355,596 L3Harris Technologies Inc 170,847 41,293,720 157,649,316 Air Freight & Logistics – 2.1% United Parcel Service Inc 704,903 128,672,994 Banks – 3.3% Bank of America Corp 1,068,412 33,

2

Janus Henderson Balanced Fund Schedule of Investments (unaudited) June 30, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 7.

Opinion and Consent of Counsel with respect to Janus Henderson Sustainable Multi-Asset Allocation Fund,

EX-99.(I)(34) 3 d391298dex99i34.htm OPINION AND CONSENT OF COUNSEL Exhibit (i)(34) August 15, 2022 Janus Investment Fund 151 Detroit Street Denver, CO 80206-4805 Re: Public Offering of Janus Henderson Sustainable Multi-Asset Allocation Fund (the “Fund”) To Whom It May Concern: I have acted as Vice President and Chief Legal Counsel for Janus Investment Fund, a Massachusetts business trust (the “Tru

August 15, 2022, is incorporated herein by reference to Exhibit (d)(65) to Post-Effective Amendment No.

Exhibit (d)(65) JANUS INVESTMENT FUND INVESTMENT ADVISORY AGREEMENT JANUS HENDERSON SUSTAINABLE MULTI-ASSET ALLOCATION FUND THIS INVESTMENT ADVISORY AGREEMENT (the ?Agreement?) is made this 15th day of August, 2022, between JANUS INVESTMENT FUND, a Massachusetts business trust (the ?Trust?), and JANUS HENDERSON INVESTORS US LLC, a Delaware limited liability company (the ?Adviser?).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM N-1A REGISTRATION STATEMENT THE SECURITIES ACT OF 1933 (No. 002-34393) ☒ Pre-Effective Amendment No. ☐ Post-Effective Amendment No. 310 ☒ REGISTRATION STATEMENT UNDER THE IN

OMB APPROVAL OMB Number: ?.? 3235-0307 Expires: September 30, 2024 Estimated average burden hours per response: 278.6 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM N-1A REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 (No. 002-34393) ? Pre-Effective Amendment No. ? Post-Effective Amendment No. 310 ? and/or REGISTRATION STATEMENT UNDER THE INVESTMENT COMPANY AC

2

CORRESP 1 filename1.htm [ Janus Henderson Investors US LLC Letterhead ] June 2, 2022 VIA EDGAR Samantha Brutlag Division of Investment Management U.S. Securities and Exchange Commission 100 F Street, N.E. Washington, DC 20549-0505 Re: JANUS INVESTMENT FUND (the “Registrant”) 1933 Act File No. 002-34393 1940 Act File No. 811-01879 Post-Effective Amendment No. 305 Dear Ms. Brutlag: This letter respo

INR

Janus Henderson Emerging Markets Managed Volatility Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Corporate Bonds– 0% Consumer Non-Cyclical – 0% Britannia Industries Ltd, 8.

2

Janus Henderson Short Duration Flexible Bond Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 33.

2

Janus Henderson Small-Mid Cap Value Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Global Allocation Fund - Conservative Schedule of Investments (unaudited) March 31, 2022 Shares Value Investment Companies£– 100.

2

Janus Henderson Global Income Managed Volatility Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Common Stocks– 95.

2

Janus Henderson Small Cap Value Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson Global Allocation Fund - Moderate Schedule of Investments (unaudited) March 31, 2022 Shares Value Investment Companies£– 100.

GBP

Janus Henderson Developed World Bond Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

AUD

Janus Henderson Absolute Return Income Opportunities Fund Schedule of Investments (unaudited) March 31, 2022 Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 6.

2

Janus Henderson Adaptive Global Allocation Fund Schedule of Investments (unaudited) March 31, 2022 Shares/Principal/ Contract Amounts Value Common Stocks– 26.

GBP

Janus Henderson Global Bond Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 1.

2

Janus Henderson Dividend & Income Builder Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Corporate Bonds– 11.

CAD

Janus Henderson Multi-Sector Income Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 53.

2

Janus Henderson U.S. Managed Volatility Fund Schedule of Investments (unaudited) March 31, 2022 Shares Value Common Stocks– 99.6% Aerospace & Defense – 1.6% Axon Enterprise Inc* 62,146 $8,559,369 L3Harris Technologies Inc 27,794 6,905,975 15,465,344 Air Freight & Logistics – 0.8% United Parcel Service Inc 34,733 7,448,839 Auto Components – 0.5% Gentex Corp 182,605 5,326,588 Banks – 0.3% Webster Fi

2

Janus Henderson Flexible Bond Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 22.

2

Janus Henderson Global Allocation Fund - Growth Schedule of Investments (unaudited) March 31, 2022 Shares Value Investment Companies£– 99.

2

Janus Henderson International Managed Volatility Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Common Stocks– 95.

2

Janus Henderson High-Yield Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 1.

2

Janus Henderson Mid Cap Value Fund Schedule of Investments (unaudited) March 31, 2022 Shares or Principal Amounts Value Common Stocks– 97.

[Janus Henderson Letterhead]

[Janus Henderson Letterhead] March 21, 2022 EDGAR Operations Branch Securities and Exchange Commission Division of Investment Management 100 F Street, N.

2

Janus Henderson Growth and Income Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 99.

2

Janus Henderson Global Equity Income Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson European Focus Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 97.

2

Janus Henderson Venture Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Global Life Sciences Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Global Sustainable Equity Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 97.

2

Janus Henderson Emerging Markets Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 96.

2

Janus Henderson Global Select Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 99.

2

Janus Henderson Forty Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Balanced Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 6.

2

Janus Henderson Contrarian Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Asia Equity Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 89.

2

Janus Henderson Research Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Global Real Estate Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Global Research Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 99.

2

Janus Henderson International Opportunities Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 94.

2

Janus Henderson Enterprise Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Global Technology and Innovation Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Overseas Fund Schedule of Investments (unaudited) December 31, 2021 Shares Value Common Stocks– 97.

2

Janus Henderson Triton Fund Schedule of Investments (unaudited) December 31, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Short-Term Bond Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 25.

2

Janus Henderson Flexible Bond Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 19.

INR

Janus Henderson Emerging Markets Managed Volatility Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Corporate Bonds– 0% Consumer Non-Cyclical – 0% Britannia Industries Ltd, 8.

2

Janus Henderson Mid Cap Value Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Common Stocks– 98.

GBP

Janus Henderson Developed World Bond Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

GBP

Janus Henderson Global Bond Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 1.

2

Janus Henderson Global Allocation Fund - Moderate Schedule of Investments (unaudited) September 30, 2021 Shares Value Investment Companies£– 100.

2

Janus Henderson Global Allocation Fund - Conservative Schedule of Investments (unaudited) September 30, 2021 Shares Value Investment Companies£– 100.

2

Janus Henderson Global Allocation Fund - Growth Schedule of Investments (unaudited) September 30, 2021 Shares Value Investment Companies£– 100.

2

Janus Henderson Small Cap Value Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Dividend & Income Builder Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Corporate Bonds– 12.

2

Janus Henderson High-Yield Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

AUD

Janus Henderson Absolute Return Income Opportunities Fund Schedule of Investments (unaudited) September 30, 2021 Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 13.

2

Janus Henderson Global Income Managed Volatility Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Small-Mid Cap Value Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson Multi-Sector Income Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 43.

2

Janus Henderson Adaptive Global Allocation Fund Schedule of Investments (unaudited) September 30, 2021 Shares/Principal/ Contract Amounts Value Common Stocks– 30.

2

Janus Henderson U.S. Managed Volatility Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Common Stocks– 99.5% Aerospace & Defense – 1.5% L3Harris Technologies Inc 78,464 $17,280,911 Auto Components – 0.2% Gentex Corp 73,006 2,407,738 Banks – 0.8% First Republic Bank/CA 44,542 8,591,261 Beverages – 0.3% Monster Beverage Corp* 43,208 3,838,167 Biotechnolo

2

Janus Henderson International Managed Volatility Fund Schedule of Investments (unaudited) September 30, 2021 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Global Technology and Innovation Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Global Sustainable Equity Fund Schedule of Investments (unaudited) June 30, 2021 Shares Value Common Stocks– 97.

2

Janus Henderson Global Select Fund Schedule of Investments (unaudited) June 30, 2021 Shares Value Common Stocks– 98.

2

Janus Henderson Emerging Markets Fund Schedule of Investments (unaudited) June 30, 2021 Shares Value Common Stocks– 96.

2

Janus Henderson Research Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Triton Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Enterprise Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Contrarian Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Global Research Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Overseas Fund Schedule of Investments (unaudited) June 30, 2021 Shares Value Common Stocks– 96.

2

Janus Henderson Asia Equity Fund Schedule of Investments (unaudited) June 30, 2021 Shares Value Common Stocks– 90.

2

Janus Henderson Growth and Income Fund Schedule of Investments (unaudited) June 30, 2021 Shares Value Common Stocks– 100.

2

Janus Henderson Global Life Sciences Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson European Focus Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson Balanced Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 5.

2

Janus Henderson International Opportunities Fund Schedule of Investments (unaudited) June 30, 2021 Shares Value Common Stocks– 96.

2

Janus Henderson Global Real Estate Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Venture Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Global Equity Income Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Forty Fund Schedule of Investments (unaudited) June 30, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Global Allocation Fund - Conservative Schedule of Investments (unaudited) March 31, 2021 Shares Value Investment Companies£– 100.

2

Janus Henderson Global Income Managed Volatility Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Global Allocation Fund - Moderate Schedule of Investments (unaudited) March 31, 2021 Shares Value Investment Companies£– 99.

2

Janus Henderson Small-Mid Cap Value Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson Dividend & Income Builder Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Corporate Bonds– 13.

2

Janus Henderson U.S. Managed Volatility Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Common Stocks– 99.7% Aerospace & Defense – 0.4% HEICO Corp 13,714 $1,725,221 L3Harris Technologies Inc 14,645 2,968,249 4,693,470 Auto Components – 0.4% Gentex Corp 132,456 4,724,706 Beverages – 0.8% Monster Beverage Corp* 98,701 8,990,674 Biotechnology – 2.7% AbbVie In

2

Janus Henderson High-Yield Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

2

Janus Henderson Short-Term Bond Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 26.

AUD

Janus Henderson Absolute Return Income Opportunities Fund Schedule of Investments (unaudited) March 31, 2021 Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 14.

INR

Janus Henderson Emerging Markets Managed Volatility Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Corporate Bonds– 0% Consumer Non-Cyclical – 0% Britannia Industries Ltd, 8.

2

Janus Henderson International Managed Volatility Fund Schedule of Investments (unaudited) March 31, 2021 Shares Value Common Stocks– 99.

2

Janus Henderson Small Cap Value Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Global Allocation Fund - Growth Schedule of Investments (unaudited) March 31, 2021 Shares Value Investment Companies£– 100.

2

Janus Henderson Adaptive Global Allocation Fund Schedule of Investments (unaudited) March 31, 2021 Shares/Principal/ Contract Amounts Value Common Stocks– 42.

2

Janus Henderson Mid Cap Value Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Common Stocks– 97.

GBP

Janus Henderson Developed World Bond Fund Schedule of Investments (unaudited) March 31, 2021 Shares/Principal/ Contract Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

2

Janus Henderson Multi-Sector Income Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 44.

GBP

Janus Henderson Global Bond Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 2.

2

Janus Henderson Flexible Bond Fund Schedule of Investments (unaudited) March 31, 2021 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 18.

* * *

May 10, 2021 David Manion U.S. Securities and Exchange Commission 100 F Street, N.E. Washington, D.C. 20549-0505 Re: JANUS ASPEN SERIES 1933 Act File No. 033-63212 1940 Act File No. 811-07736 JANUS INVESTMENT FUND 1933 Act File No. 002-34393 1940 Act File No. 811-01879 Dear Mr. Manion, On behalf of Janus Aspen Series (?JAS?) and Janus Investment Fund (?JIF,? and together with ?JAS,? the ?Registran

2

Janus Henderson Growth and Income Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 99.

2

Janus Henderson International Value Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 96.

2

Janus Henderson Contrarian Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 98.

2

Janus Henderson International Opportunities Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 95.

2

Janus Henderson Venture Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Forty Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Enterprise Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Global Research Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 100.

2

Janus Henderson Asia Equity Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 89.

2

Janus Henderson Global Real Estate Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Global Equity Income Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 92.

2

Janus Henderson Research Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 99.

2

Janus Henderson Balanced Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 5.

2

Janus Henderson Global Technology and Innovation Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Emerging Markets Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 100.

2

Janus Henderson Global Select Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 99.

2

Janus Henderson European Focus Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 97.

2

Janus Henderson Global Sustainable Equity Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 95.

2

Janus Henderson Triton Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Global Life Sciences Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 98.

2

Janus Henderson Global Value Fund Schedule of Investments (unaudited) December 31, 2020 Shares or Principal Amounts Value Common Stocks– 97.

2

Janus Henderson Overseas Fund Schedule of Investments (unaudited) December 31, 2020 Shares Value Common Stocks– 98.

GBP

Janus Henderson Developed World Bond Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

EUR

Janus Henderson High-Yield Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 0.

GBP

Janus Henderson Global Bond Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 2.

Janus Henderson Mid Cap Value Fund

Janus Henderson Mid Cap Value Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Common Stocks– 97.

Janus Henderson Adaptive Global Allocation Fund

Janus Henderson Adaptive Global Allocation Fund Schedule of Investments (unaudited) September 30, 2020 Shares/Principal/ Contract Amounts Value Common Stocks– 43.

Janus Henderson Short-Term Bond Fund

Janus Henderson Short-Term Bond Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 24.

EUR

Janus Henderson Multi-Sector Income Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 31.

Janus Henderson Global Allocation Fund - Conservative

Janus Henderson Global Allocation Fund - Conservative Schedule of Investments (unaudited) September 30, 2020 Shares Value Investment Companies£– 100.

Janus Henderson Small-Mid Cap Value Fund

Janus Henderson Small-Mid Cap Value Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Common Stocks– 96.

AUD

Janus Henderson Absolute Return Income Opportunities Fund Schedule of Investments (unaudited) September 30, 2020 Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 35.

Janus Henderson International Managed Volatility Fund

Janus Henderson International Managed Volatility Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Common Stocks– 98.

Janus Henderson Global Allocation Fund - Moderate

Janus Henderson Global Allocation Fund - Moderate Schedule of Investments (unaudited) September 30, 2020 Shares Value Investment Companies£– 100.

Janus Henderson Small Cap Value Fund

Janus Henderson Small Cap Value Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Common Stocks– 97.

Janus Henderson Large Cap Value Fund

Janus Henderson Large Cap Value Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Common Stocks– 97.

EUR

Janus Henderson Value Plus Income Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 11.

Janus Henderson Dividend & Income Builder Fund

Janus Henderson Dividend & Income Builder Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Corporate Bonds– 21.

Janus Henderson Flexible Bond Fund

Janus Henderson Flexible Bond Fund Schedule of Investments (unaudited) September 30, 2020 Shares or Principal Amounts Value Asset-Backed/Commercial Mortgage-Backed Securities– 14.